2IBI
Covalent Ubiquitin-USP2 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.839, 54.400, 75.021 |
| Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
| Resolution | 28.010 - 2.200 |
| R-factor | 0.19352 |
| Rwork | 0.190 |
| R-free | 0.25887 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2GFO 1ubq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.010 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 19694 | |
| <I/σ(I)> | 10.32 | 1.5 |
| Completeness [%] | 96.4 | 70.7 |
| Redundancy | 4.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 298 | 22% PEG1500, 0.1 M Bicine, 0.2 M NaCl, 1 mM DTT, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 9.00 |
