2IBF
Human vinculin's head domain (Vh1, residues 1-258) in complex with two vinculin binding sites of Shigella flexneri's IpaA (residues 565-587)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 154.478, 154.478, 154.478 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 109.110 - 3.200 |
R-factor | 0.2452 |
Rwork | 0.243 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.903 |
Refinement software | BUSTER-TNT (1.3.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.110 | |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 10911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |