2IAY

Crystal structure of a duf1831 family protein (lp2179) from lactobacillus plantarum at 1.20 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	36.289, 47.896, 58.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.928 - 1.200
R-factor	0.121
Rwork	0.120
R-free	0.14700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.646
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.928	28.920	1.230
High resolution limit [Å]	1.198	5.370	1.200
Rmerge	0.069	0.055	0.204
Total number of observations		3578	1663
Number of reflections	29115
<I/σ(I)>	6.1	11.4	3.7
Completeness [%]	90.4	95.2	50.7
Redundancy	6.2	9.1	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	10.5	277	0.2M NaCl, 20.0% PEG-8000, 0.1M CAPS, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 10.5
2	VAPOR DIFFUSION, SITTING DROP, NANODROP	9	277	20.0% PEG-6000, 0.1M Bicine, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 9.0