2IAM

Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	C 2 2 2
Unit cell lengths	86.391, 270.542, 97.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.910 - 2.800
R-factor	0.21051
Rwork	0.207
R-free	0.27915
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fyt 1ial
RMSD bond length	0.019
RMSD bond angle	1.960
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.940
High resolution limit [Å]	2.800	2.800
Rmerge	0.091	0.513
Number of reflections	26581
<I/σ(I)>	10.7	3
Completeness [%]	92.8	91.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	8% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K