2IA5
T4 polynucleotide kinase/phosphatase with bound sulfate and magnesium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 124.630, 128.050, 357.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1LTQ dimer |
| RMSD bond length | 0.151 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 124693 | |
| <I/σ(I)> | 12.01 | 1.57 |
| Completeness [%] | 98.7 | |
| Redundancy | 4.32 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 295 | 100mM Sodium cacodylate, 12% PEG-8000, 0.2 M ammonium sulfate, 20 mM urea, 5 mM DTT, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
