2I9B
Crystal structure of ATF-urokinase receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-07 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.914, 281.921, 62.811 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.22372 |
| Rwork | 0.222 |
| R-free | 0.26498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ywh 2i9a |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.888 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 47048 | |
| <I/σ(I)> | 17.6 | 2.44 |
| Completeness [%] | 91.2 | 51.3 |
| Redundancy | 2.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 22.5% w/v PEG3350, 200 mM ammonium sulfate, 100 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
