2I9A
Crystal structure of the free aminoterminal fragment of urokinase type plasminogen activator (ATF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-11 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 1.13539 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.603, 64.327, 64.623 |
| Unit cell angles | 107.62, 92.11, 95.75 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.1829 |
| Rwork | 0.182 |
| R-free | 0.20822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urk |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 54650 | |
| <I/σ(I)> | 19.63 | 2.58 |
| Completeness [%] | 96.4 | 87 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 293 | 1.2 M sodium dihydrogen phosphate, 0.8 M potassium hydrogen phosphate, 200 mM lithium sulfate, 100 mM CHES, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
