2I62
Mouse Nicotinamide N-methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.281, 158.519, 72.227 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 79.310 - 1.800 |
| R-factor | 0.19833 |
| Rwork | 0.196 |
| R-free | 0.24632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.457 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.068 |
| Number of reflections | 85746 |
| Completeness [%] | 81.7 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 300 | PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
