2I5D
Crystal Structure of Human Inosine Triphosphate Pyrophosphatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 31.126, 104.990, 49.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.840 - 1.630 |
Rwork | 0.190 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2car |
RMSD bond length | 0.013 |
RMSD bond angle | 1.391 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.054 | 0.395 |
Number of reflections | 20838 | |
<I/σ(I)> | 35.7 | 3.3 |
Completeness [%] | 98.1 | 98.2 |
Redundancy | 5.9 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 27% PEG 3350 100mM HEPES pH 7.5, VAPOR DIFFUSION, temperature 293K |