2I54

Phosphomannomutase from Leishmania mexicana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Collection date	2005-04-06
Wavelength(s)	0.979417, 0.979564, 0.984011
Spacegroup name	P 31 2 1
Unit cell lengths	92.413, 92.413, 173.249
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.800 - 2.100
Rwork	0.189
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.460
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100	2.100
Rmerge		0.518
Number of reflections	50609
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	14% (w/v) PEG3350, 10% PEG 400, 0.1M citrate, 50% PEG3550, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K