2I35
Crystal structure of rhombohedral crystal form of ground-state rhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2004-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 277.534, 277.534, 66.321 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.800 |
R-factor | 0.37542 |
Rwork | 0.373 |
R-free | 0.41841 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hzx |
RMSD bond length | 0.009 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.092 | |
Number of reflections | 8204 | |
<I/σ(I)> | 22.3 | 1.3 |
Completeness [%] | 84.5 | 59.2 |
Redundancy | 15.9 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 278 | 80 mM MES, pH 6.3, 110 mM N-nonyl-beta-D-glucoside, 200 mM zinc acetate, 16 mM beta-mercaptoethanol, 0.4% Merpol DA, 0.1% sodium azide, equilibrated against 3.0 M ammonium sulfate, VAPOR DIFFUSION, temperature 278K |