2I26
Crystal structure analysis of the nurse shark new antigen receptor ancestral variable domain in complex with lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.300, 64.694, 159.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.640 - 2.500 |
| R-factor | 0.20381 |
| Rwork | 0.199 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.846 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 23397 | |
| <I/σ(I)> | 8.7 | 2.9 |
| Completeness [%] | 98.1 | 97.3 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 295 | 11% PEG 4000, 1.8M ammonium sulfate, 0.1M BES, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
