2I20
Bacteriorhodopsin/lipid complex, M state of D96A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-08 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.97977 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.021, 61.021, 110.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.080 |
| R-factor | 0.156 |
| Rwork | 0.153 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.055 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.170 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.066 | 0.625 |
| Number of reflections | 13966 | |
| <I/σ(I)> | 23.4 | 3.8 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CUBIC LIPID PHASE | 8.5 | 295 | MO:WATER:PHOSPHATE, pH 8.50, CUBIC LIPID PHASE, temperature 295K |
