2I1Y
Crystal structure of the phosphatase domain of human PTP IA-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93971 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.907, 74.229, 121.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 2.230 |
R-factor | 0.20123 |
Rwork | 0.199 |
R-free | 0.26698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fh7 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.146 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.230 | 2.230 |
Rmerge | 0.225 | |
Number of reflections | 32726 | |
<I/σ(I)> | 2.7 | 0.5 |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 6.8 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 25% PEG3350, 100 MM BIS-TRIS, 200 MM AMMONIUM ACETATE, PH 6.0, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 294K |