2I1W
Crystal structure of NAD kinase 1 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Collection date | 2004-05-05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.612, 119.017, 67.937 |
| Unit cell angles | 90.00, 102.08, 90.00 |
Refinement procedure
| Resolution | 34.370 - 2.340 |
| R-factor | 0.19607 |
| Rwork | 0.194 |
| R-free | 0.25696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u0r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.374 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.370 |
| High resolution limit [Å] | 2.340 |
| Rmerge | 0.066 |
| Number of reflections | 42609 |
| <I/σ(I)> | 13.9 |
| Completeness [%] | 97.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 0.3 M potassium chloride, 50 mM tri-sodium citrate, 15-20% w/v polyethylene glycol 400, 10 mM KI, pH 5.4, VAPOR DIFFUSION, HANGING DROP, pH 5.40 |
