2I0Y
cFMS tyrosine kinase (FGF KID) in complex with an arylamide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.99997 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.070, 81.070, 144.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.100 - 1.900 |
| R-factor | 0.246 |
| Rwork | 0.238 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2000) |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.100 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 32580 | |
| <I/σ(I)> | 12.7 | 3 |
| Completeness [%] | 97.4 | 92.1 |
| Redundancy | 4.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | PEG 3350, sodium acetate, Li2SO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
