2I0Q
Crystal structure of a telomere single-strand DNA-protein complex from O. nova with full-length alpha and beta telomere proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.331, 93.331, 423.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 85120 | |
| <I/σ(I)> | 38.8 | 3.5 |
| Completeness [%] | 98.9 | 95.7 |
| Redundancy | 8.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 277.15 | 21% (w/v) PEG-4000, 10% (v/v) ethylene glycol, 1.5 M sodium chloride, 40 mM MES, 2 mM DTT, 0.02% sodium azide, VAPOR DIFFUSION, HANGING DROP, pH 5.75, temperature 277.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG-4000 | ||
| 10 | 1 | 2 | sodium azide | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | ethylene glycol | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | sodium azide | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | PEG-4000 | ||
| 9 | 1 | 2 | sodium chloride |
