2I0C
Crystal structure of the GluR6 ligand binding core dimer crosslinked by disulfide bonds between Y490C and L752C at 2.25 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 4 |
| Unit cell lengths | 108.790, 108.790, 47.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.250 |
| R-factor | 0.17528 |
| Rwork | 0.172 |
| R-free | 0.24447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 17SY |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.977 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.089 | 0.437 |
| Number of reflections | 26406 | |
| <I/σ(I)> | 10.1 | 2.93 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | Reservoir: 20% isopropanol, 25% PEG 4k, 0.1 M NaCitrate. Protein: 7-15 mg/ml, 10 mM HEPES, 20 mM NaCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
