2HZV

NikR-operator DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	197.203, 76.132, 132.092
Unit cell angles	90.00, 110.27, 90.00

Refinement procedure

Resolution	49.280 - 3.100
R-factor	0.261
Rwork	0.261
R-free	0.30300
Structure solution method	Molecular Replacement/SAD
Starting model (for MR)	1q5y 1q5v
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	3.210
High resolution limit [Å]	3.100	3.100
Number of reflections	30232
Completeness [%]	94.1	70.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	200 mM KCl, 50 mM MgCl2, 50 mM Tris pH 7.5, and 10% w/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	KCl
2	1	1	MgCl2
3	1	1	Tris
4	1	1	PEG 4000
5	1	2	PEG 4000
6	1	2	MgCl2
7	1	2	KCl