2HZL
Crystal structures of a sodium-alpha-keto acid binding subunit from a TRAP transporter in its closed forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.021, 78.057, 95.139 |
| Unit cell angles | 90.00, 124.98, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.400 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hzk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.143 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.410 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 137318 | |
| Completeness [%] | 99.0 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 100mM sodium citrate 1.4M ammonium sulfate 30mM pyruvate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
