2HW8
Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-22 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.843 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 67.870, 67.870, 340.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zho |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.220 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.075 | 0.342 |
Number of reflections | 28370 | |
<I/σ(I)> | 14.9 | 4.3 |
Completeness [%] | 99.3 | 97.1 |
Redundancy | 6.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG1000, sodium cacodylate,0.5% glycerol, 0.2 mM HgCl2 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG1000 | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | HgCl2 | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG1000 | ||
7 | 1 | 2 | sodium cacodylate | ||
8 | 1 | 2 | glycerol | ||
9 | 1 | 2 | HgCl2 |