2HW8
Structure of ribosomal protein L1-mRNA complex at 2.1 resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.843 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 67.870, 67.870, 340.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zho |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | 0.342 |
| Number of reflections | 28370 | |
| <I/σ(I)> | 14.9 | 4.3 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG1000, sodium cacodylate,0.5% glycerol, 0.2 mM HgCl2 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1000 | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | HgCl2 | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG1000 | ||
| 7 | 1 | 2 | sodium cacodylate | ||
| 8 | 1 | 2 | glycerol | ||
| 9 | 1 | 2 | HgCl2 |
