2HVQ
Structure of Adenylated full-length T4 RNA Ligase 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.020, 57.850, 119.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.560 - 2.400 |
Rwork | 0.211 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s68 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.560 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.120 | 0.382 |
Number of reflections | 12520 | |
<I/σ(I)> | 7.4 | 3.2 |
Completeness [%] | 91.6 | 89.6 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 291 | 100 mM MES, 10% MPD, 30 mM NaCl, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K |