2HVC
The Crystal Structure of Ligand-binding Domain (LBD) of human Androgen Receptor in Complex with a selective modulator LGD2226
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 79 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.640, 65.390, 72.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.251 |
| Rwork | 0.221 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xq3 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.055 | 0.283 |
| Number of reflections | 16570 | |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 11.9 | 3.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 289 | mixing the protein solution(5mg/ml) with equal volume of the reservoir solution with 0.4M Na2HPO4, 0.4M K2HPO4, as the primary precipitators. pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
