2HTK
Structure of the Escherichia coli ClC chloride channel Y445A mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.067, 98.139, 171.068 |
| Unit cell angles | 90.00, 131.56, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.410 |
| R-factor | 0.2663 |
| Rwork | 0.266 |
| R-free | 0.27609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ots |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.958 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.073 | |
| Number of reflections | 36035 | |
| Completeness [%] | 97.0 | 75.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 41% peg 300 w/v, 50mM Tris, 150mM NaKTart, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
