2HT4
Structure of the Escherichia coli ClC chloride channel Y445W mutant and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9193 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.007, 95.940, 175.327 |
| Unit cell angles | 90.00, 133.20, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.24049 |
| Rwork | 0.239 |
| R-free | 0.26106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.589 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.088 | |
| Number of reflections | 45398 | |
| Completeness [%] | 96.9 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 28% peg 300 v/v, 50 mM HEPES, 100mM NaBr, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
