2HPC

Crystal structure of fragment D from Human Fibrinogen Complexed with Gly-Pro-Arg-Pro-amide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95
Spacegroup name	P 1 2 1
Unit cell lengths	81.677, 46.069, 429.901
Unit cell angles	90.00, 89.99, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.2792
Rwork	0.275
R-free	0.36020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hod
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	91090
Completeness [%]	88.9
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	equal volumes of 10 mg/ml fragment D, 5 mM Gly-Pro-Arg-Pro-amide, 50 mM Tris, pH 7.0 and 16% PEG 3350, 50 mM Tris pH 8.0, 20 mM CaCl2, 2 mM sodium azide., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K