2HP3
Crystal structure of iminodisuccinate epimerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0628 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.365, 104.210, 78.545 |
| Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.710 |
| R-factor | 0.15893 |
| Rwork | 0.157 |
| R-free | 0.19155 |
| Structure solution method | MIR |
| Starting model (for MR) | 2hp0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 92811 | |
| <I/σ(I)> | 14.3 | 3.4 |
| Completeness [%] | 97.6 | 84.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.2 | 298 | 22 % PEG 3350, 0.2M ammonium sulphate, 0.1M Bis-Tris propane, pH 7.2, VAPOR DIFFUSION, temperature 298K |
