2HNL
Structure of the prostaglandin D synthase from the parasitic nematode Onchocerca volvulus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Collection date | 2004-12-22 |
Wavelength(s) | 0.803 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.783, 90.995, 106.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1PD2 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.716 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.069 | 0.031 | 0.360 |
Number of reflections | 34249 | ||
<I/σ(I)> | 15.1 | ||
Completeness [%] | 99.7 | 99 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Reservoir: 22% PEG4000, 10% Isopropanol, 0.1M Hepes pH 7.5 Protein stock: 5.5mg/ml in 100mM Na-acetate-trihydrate pH 8.0, temperature 298K, VAPOR DIFFUSION, HANGING DROP |