2HMH
Crystal structure of SOCS3 in complex with gp130(pTyr757) phosphopeptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Collection date | 2006-03-11 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.918, 94.708, 69.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.350 - 2.000 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2SHP |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.560 | 0.323 |
| Number of reflections | 9956 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 297 | 30% PEG8000, 0.1 M Sodium Cacodylate, 0.2 M Sodium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 297K, pH 6.50 |
