2HMA
The Crystal Structure of tRNA (5-Methylaminomethyl-2-Thiouridylate)-Methyltransferase TrmU from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.319, 93.319, 157.018 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.730 - 2.410 |
| R-factor | 0.19315 |
| Rwork | 0.190 |
| R-free | 0.21661 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.498 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.730 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.127 | 0.578 |
| Number of reflections | 31670 | |
| <I/σ(I)> | 11.8 | 2.78 |
| Completeness [%] | 98.7 | 88.7 |
| Redundancy | 7.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 100mM Tris pH=8.5, and 3.5M NaFormate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
