2HKO
Crystal structure of LSD1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1, 1.00696, 1.00874 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 187.113, 187.113, 106.581 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.28300 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.350 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.085 | 0.632 |
Number of reflections | 26719 | |
<I/σ(I)> | 1.63 | |
Completeness [%] | 96.0 | 73.4 |
Redundancy | 17.3 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.3 | 289 | 5% PEG 8K, 10 mM MgCl2, 150 mM NaCl, 50 mM Na2HPO4/KH2PO4 pH 6.33, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 289K, pH 6.30 | |
1 | 6.3 | 289 | 5% PEG 8K, 10 mM MgCl2, 150 mM NaCl, 50 mM Na2HPO4/KH2PO4 pH 6.33, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 289K, pH 6.30 |