2HI9
Crystal Structure of human native protein C inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.590, 70.950, 123.880 |
| Unit cell angles | 90.00, 127.92, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.083 | 0.396 |
| Number of reflections | 60523 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 66.7mM tri-potassium citrate, 6.7% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
