2HI8
human formylglycine generating enzyme, C336S mutant, bromide co-crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.81 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.158, 108.792, 43.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.200 - 1.640 |
R-factor | 0.15516 |
Rwork | 0.153 |
R-free | 0.19067 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2afy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 35818 | |
<I/σ(I)> | 15 | 3 |
Completeness [%] | 97.7 | 78.2 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000, Calcium chloride, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |