2HHZ

Crystal structure of a pyridoxamine 5'-phosphate oxidase-related protein (ssuidraft_2804) from streptococcus suis 89/1591 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-04
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97934, 0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	71.000, 71.000, 58.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.198 - 2.000
R-factor	0.19276
Rwork	0.191
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.198	29.198	2.070
High resolution limit [Å]	2.000	3.420	2.000
Rmerge	0.085	0.050	0.726
Number of reflections	10580
<I/σ(I)>	9.48	20	1.9
Completeness [%]	94.4	99.5	81.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	4.5	277	40.0% PEG-400, 0.1M Acetate pH 4.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K