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2HHP

Structure of yeast poly(A) polymerase in a closed conformation.

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X29A
Synchrotron siteNSLS
BeamlineX29A
Temperature [K]100
Detector technologyCCD
Collection date2005-03-14
DetectorADSC QUANTUM 315
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths58.483, 84.918, 67.721
Unit cell angles90.00, 111.78, 90.00
Refinement procedure
Resolution33.040 - 1.800
R-factor0.19849
Rwork0.197
R-free0.23551
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1fa0
RMSD bond length0.017
RMSD bond angle1.564
Refinement softwareREFMAC (5.2.0003)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.860
High resolution limit [Å]1.8001.800
Rmerge0.448
Number of reflections56901
<I/σ(I)>463.46
Completeness [%]99.798.3
Redundancy6.95.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.6The crystals were grown by combining the protein at 20mg/ml with an equal volume of 20% isopropanol, 20% Peg 4000, 100mM Citrate pH 5.6, and 0.1% beta mercaptoethanol, and then leaving the hanging drops for two weeks over an empty reservoir well., vapor diffusion, hanging drop

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