2HHJ
Human bisphosphoglycerate mutase complexed with 2,3-bisphosphoglycerate (15 days)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-19 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.692, 71.555, 159.194 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.830 - 1.500 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.069 | 0.438 |
Number of reflections | 88158 | |
<I/σ(I)> | 19.6 | 1.76 |
Completeness [%] | 97.8 | 92.3 |
Redundancy | 4.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG6000, TEMPERATURE 289K, pH 7.50, VAPOR DIFFUSION, HANGING DROP |