2HFZ
Crystal structure of RNA dependent RNA polymerase domain from West Nile virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-18 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 73.333, 103.449, 190.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.350 - 3.000 |
R-factor | 0.26 |
Rwork | 0.260 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hcs |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT (1.3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.770 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.337 | |
Number of reflections | 14328 | |
<I/σ(I)> | 12.9 | 3.4 |
Completeness [%] | 96.4 | 100 |
Redundancy | 4.7 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 5-25% PEG 1000 0.1 M NaCacodylate 0.3 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00 |