2HF3
Crystal structure of monomeric Actin in the ADP bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-08 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.700, 54.070, 39.590 |
| Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
| Resolution | 19.190 - 1.800 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j6z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 38620 | |
| <I/σ(I)> | 15.1 | 2.1 |
| Completeness [%] | 98.4 | 86.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 277 | 25% MPD, 0.1M NaAcetate, 0.1M NaCl, 0.02M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 5.40 |
