2HET
Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.889, 70.759, 85.925 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 70.000 - 3.000 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.044 | 0.234 |
| Number of reflections | 14380 | |
| <I/σ(I)> | 11.3 | 1.7 |
| Completeness [%] | 84.8 | 84.1 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 2.4 M sodium malonate, 2 mM calcium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
