2HEH

Crystal Structure of the KIF2C motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-17
Detector	ADSC QUANTUM 210
Spacegroup name	P 43 21 2
Unit cell lengths	79.720, 79.720, 154.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.22126
Rwork	0.220
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v8k
RMSD bond length	0.018
RMSD bond angle	1.368
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.077	0.713
Number of reflections	51791
<I/σ(I)>	9.9
Completeness [%]	99.8
Redundancy	7.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	291	37% PEG400, 0.2M NaCl, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 7.00