2HDN

Trypsin-modified Elongation Factor Tu in complex with tetracycline at 2.8 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	P 1 21 1
Unit cell lengths	69.710, 156.060, 134.830
Unit cell angles	90.00, 95.38, 90.00

Refinement procedure

Resolution	40.000 - 2.800
Rwork	0.180
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	an unrefined 2.7 Angstrom model of E. coli trypsin-modified EF-Tu-MgGDP consisting of residues 9 to 40 50 to 258 and 260 to 393 plus one Mg ion and GDP
RMSD bond length	0.020
RMSD bond angle	1.820
Phasing software	MERLOT
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	47.300
High resolution limit [Å]	2.700
Rmerge	0.099
Number of reflections	73085
Completeness [%]	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			Multiple crystals were used to collect the native and each derivative data set. VAPOR DIFFUSION, SITTING DROP