2HDK
Crystal Structure of Cys315Ala-Cys318Ala Mutant of Human Mitochondrial Branched Chain Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91609 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.480, 112.187, 60.265 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.32 |
| Rwork | 0.182 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.107 | 0.041 | 0.656 |
| Number of reflections | 28489 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 93.2 | 84.3 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 22 to 30 % PEG1500, 100mM HEPES, 20mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
