2HCT
Acidic residues at the active sites of CD38 and ADP-ribosyl cyclase determine NAAPD synthesis and hydrolysis activities
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 |
Unit cell lengths | 41.715, 52.823, 65.578 |
Unit cell angles | 106.26, 91.85, 95.25 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.19807 |
Rwork | 0.195 |
R-free | 0.24755 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yh3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.459 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 37216 | |
Completeness [%] | 97.1 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM MES, 12% PEG 4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |