2HCS
Crystal structure of RNA dependant RNA polymerase domain of West Nile virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 43 |
| Unit cell lengths | 109.991, 109.991, 69.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.880 - 2.500 |
| R-factor | 0.20779 |
| Rwork | 0.206 |
| R-free | 0.24316 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.723 |
| Data reduction software | MOSFLM |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 28709 | |
| <I/σ(I)> | 18.4 | 2.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 5% PEG 10000, 0.2 M Imidazole Malate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
