2HBL
Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn, Zn, and AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-5 |
Synchrotron site | MAX II |
Beamline | I911-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-19 |
Detector | MARRESEARCH |
Wavelength(s) | 1.043 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.846, 110.846, 79.873 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.870 - 2.300 |
R-factor | 0.243 |
Rwork | 0.239 |
R-free | 0.29000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2hbj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.238 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.870 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.053 | 0.726 |
Number of reflections | 25647 | |
<I/σ(I)> | 11.3 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |