2HB2
Structure of HIV protease 6X mutant in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97944 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 49.671, 49.671, 109.613 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.218 - 2.300 |
R-factor | 0.291 |
Rwork | 0.234 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.093 | 0.041 |
Number of reflections | 6506 | |
<I/σ(I)> | 13.7 | 1.2 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 281.16 | 5% PEG 3350, 0.05M Na/K Tartrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 281.16K |