2HAN
Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8115 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.716, 59.790, 65.179 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 26.920 - 1.950 |
| R-factor | 0.1816 |
| Rwork | 0.180 |
| R-free | 0.21706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r0o |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.029 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.549 |
| Number of reflections | 25368 | |
| <I/σ(I)> | 24.2 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 21% PEG 3350, 0.1 M NaCl, 0.1 M MES, 1 mM DTT, 5 mikroM ZnCl2, 0.1 M LiCl, 10 mM MgCl2 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 10 | 1 | 2 | NaCl | ||
| 11 | 1 | 2 | ZnCl2 | ||
| 12 | 1 | 2 | LiCl | ||
| 13 | 1 | 2 | MgCl2 | ||
| 14 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 1 | ZnCl2 | ||
| 6 | 1 | 1 | LiCl | ||
| 7 | 1 | 1 | MgCl2 | ||
| 8 | 1 | 1 | H2O | ||
| 9 | 1 | 2 | PEG 3350 |
