2H8R
Hepatocyte Nuclear Factor 1b bound to DNA: MODY5 Gene Product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97923 |
| Spacegroup name | H 3 |
| Unit cell lengths | 174.689, 174.689, 72.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.22614 |
| Rwork | 0.223 |
| R-free | 0.28990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic8 |
| RMSD bond length | 0.079 |
| RMSD bond angle | 5.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.900 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.088 | 0.313 |
| Number of reflections | 12582 | |
| <I/σ(I)> | 28.8 | 3.35 |
| Completeness [%] | 93.9 | 63.4 |
| Redundancy | 6.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 6%(v/v) PEG300, 5%(v/v) PEG8000, 8%(v/v) glycerol, 100mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG300 | ||
| 2 | 1 | 1 | PEG8000 | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | Tris | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG300 | ||
| 7 | 1 | 2 | PEG8000 | ||
| 8 | 1 | 2 | Tris | ||
| 9 | 1 | 2 | H2O |
