2H8F
Crystal structure of deoxy hemoglobin from Trematomus bernacchii at pH 6.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-10-10 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.850, 95.042, 61.824 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 19.830 - 1.300 |
R-factor | 0.151 |
Rwork | 0.151 |
R-free | 0.17200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 3.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.830 | 1.460 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 150761 | |
Completeness [%] | 86.3 | 57.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | deoxy-HbTb 8-9.5 mg/ml mixed with equal amount of 7-12 % w/v PEG 6000, PBS 100 mM, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |