2H5E

Crystal structure of E.coli polypeptide release factor RF3

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-16
Detector	MAR CCD 130 mm
Wavelength(s)	0.9788, 0.9795, 0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	73.174, 239.781, 69.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.225
R-free	0.26200
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.252
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.068
Number of reflections	29229
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7%-12% (w/v) PEG 3350, 0.02M di-Ammonium hydrogen citrate, 10% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K